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Molecular Plant Advance Access originally published online on July 21, 2008
Molecular Plant 2008 1(5):770-785; doi:10.1093/mp/ssn035
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© The Author 2008. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS.

A Novel C2-Domain Phospholipid-Binding Protein, OsPBP1, Is Required for Pollen Fertility in Rice

Wen-Qiang Yanga,b, Ying Laia,b, Mei-Na Lia,b, Wen-Ying Xua and Yong-Biao Xuea,1

a Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Centre for Plant Gene Research, Beijing 100190, China
b Graduate School of Chinese Academy of Sciences, Beijing 100049, China

1 To whom correspondence should be addressed. E-mail ybxue{at}genetics.ac.cn, fax +86-10–6253 7814, tel. +86-10–6255 2880.

Pollen fertility is a crucial factor for successful pollination and essential for seed formation. Recent studies have suggested that a diverse range of internal and external factors, signaling components and their related pathways are likely involved in pollen fertility. Here, we report a single C2-domain containing protein, OsPBP1, initially identified through cDNA microarray analysis. OsPBP1 is a single copy gene and preferentially expressed in pistil and pollen but down-regulated by pollination. OsPBP1 had a calcium concentration-dependent phospholipid-binding activity and was localized mainly in cytoplasm and nucleus, but translocated onto the plasma membrane in response to an intracellular Ca2+ increase. Pollen grains of antisense OsPBP1 transgenic lines were largely nonviable, germinated poorly in vitro and of low fertility. OsPBP1 protein was localized in a region peripheral to pollen wall and vesicles of elongating pollen tube, and its repressed expression reduced substantially this association and led to alteration of microfilament polymerization during pollen germination. Taken together, these results indicate that OsPBP1 is a novel functional C2-domain phospholipids-binding protein that is required for pollen fertility likely by regulating Ca2+ and phospholipid signaling pathways.

Key Words: rice (Oryza sativa L.) • pollination • pollen fertility • OsPBP1 • C2-domain • phospholipid


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