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Molecular Plant Advance Access originally published online on September 26, 2008
Molecular Plant 2008 1(6):950-960; doi:10.1093/mp/ssn054
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© The Author 2008. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS.

Mitotic Spindle Organization by the Preprophase Band

J. Christian Ambrosea,b and Richard Cyra,1

a The Pennsylvania State University, Department of Biology, The Huck Institutes of the Life Sciences, Integrative Biosciences Graduate Degree Program, Plant Physiology Program, University Park, PA 16802, USA
b Present address: Department of Botany, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada

1 To whom correspondence should be addressed, to 208 Mueller Lab at address a. E-mail rjc8{at}psu.edu, fax (814) 865-9131, tel. (814) 863-8618.

In higher plants, the preprophase band (PPB) of microtubules (MTs) forecasts the cell division site prior to mitosis and specifies the organization of MTs into a bipolar prophase spindle surrounding the nucleus. However, the mechanisms governing this PPB-dependent establishment of bipolarity are unclear. Here, we present evidence from live cell imaging studies that suggest a role for the MTs bridging the PPB and the prophase nucleus in mediating this function. Results from drug treatments, along with genetic evidence from null kinesin plants, suggest that these MTs contribute to the bipolarity, orientation, and position of the prophase spindle. Specifically, the absence of these bridge MTs is associated with lack of bipolarity, while non-uniform distributions of bridge MTs correlate with prophase spindle migration, deformation, and enhanced bipolarity toward the region of highest bridge MT density. This behavior does not require actomyosin-based forces, and is enhanced by suppressing MT dynamics with taxol. These observations occur during late prophase, and are coincident with the gradual closing of annular spindle poles. Based on these data, we describe a hypothetical mechanism for bridge MT-dependent organization of prophase spindles.

Key Words: preprophase band • mitosis • acentrosomal spindle • microtubule • kinesin • ATK5 • cytokinesis


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