Skip Navigation

Molecular Plant 2009 2(1):152-165; doi:10.1093/mp/ssn095
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kumar, M.
Right arrow Articles by Schöffl, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2009. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS.

Heat Shock Factors HsfB1 and HsfB2b Are Involved in the Regulation of Pdf1.2 Expression and Pathogen Resistance in Arabidopsis

Mukesh Kumara, Wolfgang Buschb, Hannah Birkec, Birgit Kemmerlingd, Thorsten Nürnbergerd and Friedrich Schöfflc,1

a Present address: Heinrich-Pette-Institut, Martinistrabe 52, D-20251 Hamburg, Germany
b Max-Planck-Institut für Entwicklungsbiologie, Abteilung Molekularbiologie, Speemannstrabe 37–39, D-72076 Tübingen, Germany
c Universität Tübingen, Zentrum für Molekularbiologie der Pflanzen (ZMBP)—Allgemeine Genetik, Auf der Morgenstelle 28, D-72076 Tübingen, Germany
d Universität Tübingen, Zentrum für Molekularbiologie der Pflanzen (ZMBP)—Biochemie der Pflanzen, Auf der Morgenstelle 5, D-72076 Tübingen, Germany

1 To whom correspondence should be addressed. E-mail friedrich.schoeffl{at}zmbp.uni-tuebingen.de, fax +49-7071-295042, tel. +49-7071-2978831.

In order to assess the functional roles of heat stress-induced class B-heat shock factors in Arabidopsis, we investigated T-DNA knockout mutants of AtHsfB1 and AtHsfB2b. Micorarray analysis of double knockout hsfB1/hsfB2b plants revealed as strong an up-regulation of the basal mRNA-levels of the defensin genes Pdf1.2a/b in mutant plants. The Pdf expression was further enhanced by jasmonic acid treatment or infection with the necrotrophic fungus Alternaria brassicicola. The single mutant hsfB2b and the double mutant hsfB1/B2b were significantly improved in disease resistance after A. brassicicola infection. There was no indication for a direct interaction of Hsf with the promoter of Pdf1.2, which is devoid of perfect HSE consensus Hsf-binding sequences. However, changes in the formation of late HsfA2-dependent HSE binding were detected in hsfB1/B2b plants. This suggests that HsfB1/B2b may interact with class A-Hsf in regulating the shut-off of the heat shock response. The identification of Pdf genes as targets of Hsf-dependent negative regulation is the first evidence for an interconnection of Hsf in the regulation of biotic and abiotic responses.

Key Words: Abiotic/environmental stress • transcriptional control and transcription factors • transcriptome analysis • defense responses • disease resistance • Arabidopsis


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.