Skip Navigation


Molecular Plant Advance Access originally published online on June 15, 2009
Molecular Plant 2009 2(4):832-837; doi:10.1093/mp/ssp038
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Data
Right arrow All Versions of this Article:
2/4/832    most recent
ssp038v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Zheng, Y.
Right arrow Articles by Shou, H.-X.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2009. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS.

Expression of the Arabidopsis thaliana Histone Gene AtHTA1 Enhances Rice Transformation Efficiency

Ye Zhenga,2, Xiao-Wei Hea,2, Ying-Hui Yinga, Jiang-Feng Lua, Stanton B. Gelvinb and Hui-Xia Shoua,1

a State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Yuhangtanghe Road 368, Hangzhou 310058, China
b Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392, USA

1 To whom correspondence should be addressed. E-mail: huixia{at}zju.edu.cn, fax 86-0571-88206133, tel. 86-0571-88206146.

We expressed the Arabidopsis thaliana histone AtHTA1 in rice under the control of the maize ubiquitin promoter. Transformation efficiencies of rice plants that constitutively expressed AtHTA1 were 28–44% higher than calli containing an empty vector control. Furthermore, co-infection of rice calli with a vector containing AtHTA1 and another vector with the target gene increased transformation by 27–50%. Thus, expression of AtHTA1 either transiently or in stably transformed cells improved rice transformation efficiency.

Key Words: Arabidopsis • histone H2A • Agrobacterium • rice • transformation

2 These authors contributed equally to this work.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.