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Molecular Plant Advance Access originally published online on July 7, 2009
Molecular Plant 2009 2(5):1067-1083; doi:10.1093/mp/ssp045
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© The Author 2009. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS.

Analysis of Transcriptome Changes Induced by Ptr ToxA in Wheat Provides Insights into the Mechanisms of Plant Susceptibility

Iovanna Pandelova, Melania F. Betts, Viola A. Manning, Larry J. Wilhelm, Todd C. Mockler and Lynda M. Ciuffetti1

Department of Botany and Plant Pathology and Center for Genome Research and Biocomputing, Oregon State University, Corvallis, OR 97331, USA

1 To whom correspondence should be addressed at Department of Botany and Plant Pathology, Cordley Hall 2082, Oregon State University, Corvallis, Oregon 97331–2902, USA. E-mail ciuffetL{at}science.oregonstate.edu, fax (541) 737-3573, tel. (541) 737-2188.

To obtain greater insight into the molecular events underlying plant disease susceptibility, we studied transcriptome changes induced by a host-selective toxin of Pyrenophora tritici-repentis, Ptr ToxA (ToxA), on its host plant, wheat. Transcriptional profiling of ToxA-treated leaves of a ToxA-sensitive wheat cultivar was performed using the GeneChip® Wheat Genome Array. An improved and up-to-date annotation of the wheat microarray was generated and a new tool for array data analysis (BRAT) was developed, and both are available for public use via a web-based interface. Our data indicate that massive transcriptional reprogramming occurs due to ToxA treatment, including cellular responses typically associated with defense. In addition, this study supports previous results indicating that ToxA-induced cell death is triggered by impairment of the photosynthetic machinery and accumulation of reactive oxygen species. Based on results of this study, we propose that ToxA acts as both an elicitor and a virulence factor.

Key Words: Gene expression • transcriptome analysis • defense responses • fungal pathogenesis • plant–microbe interactions • host-selective toxin


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