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Molecular Plant Advance Access originally published online on June 19, 2009
Molecular Plant 2009 2(5):1084-1094; doi:10.1093/mp/ssp033
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© The Author 2009. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS.

Virus-Induced Gene Silencing in the Culinary Ginger (Zingiber officinale): An Effective Mechanism for Down-Regulating Gene Expression in Tropical Monocots

Tanya Renner, Jennifer Bragga, Heather E. Driscoll, Juliana Cho, Andrew O. Jackson and Chelsea D. Specht1

Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA
a Present address: Genomics and Gene Discovery, USDA, ARS, PWA, WRRC-GGD, 800 Buchanan Street, Albany, CA 94710, USA

1 To whom correspondence should be addressed at 111 Koshland Hall, MC 3102, Berkeley, CA 94720, USA. E-mail cdspecht{at}nature.berkeley.edu, fax 510-642-4995, tel. 510-642-5601.

Virus-induced gene silencing (VIGS) has been shown to be effective for transient knockdown of gene expression in plants to analyze the effects of specific genes in development and stress-related responses. VIGS is well established for studies of model systems and crops within the Solanaceae, Brassicaceae, Leguminaceae, and Poaceae, but only recently has been applied to plants residing outside these families. Here, we have demonstrated that barley stripe mosaic virus (BSMV) can infect two species within the Zingiberaceae, and that BSMV–VIGS can be applied to specifically down-regulate phytoene desaturase in the culinary ginger Zingiber officinale. These results suggest that extension of BSMV–VIGS to monocots other than cereals has the potential for directed genetic analyses of many important temperate and tropical crop species.

Key Words: Barley stripe mosaic virus • virus-induced gene silencing • VIGS • Zingiber officinale • Monocot


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