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Molecular Plant Advance Access originally published online on October 20, 2009
Molecular Plant 2009 2(6):1289-1297; doi:10.1093/mp/ssp075
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© The Author 2009. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS.

Pitt, a Novel Tetratricopeptide Repeat Protein Involved in Light-Dependent Chlorophyll Biosynthesis and Thylakoid Membrane Biogenesis in Synechocystis sp. PCC 6803

Marco Schottkowskia, Janina Ratkea, Ulrike Osterb, Marc Nowaczykc and Jörg Nickelsena,1

a Molekulare Pflanzenwissenschaften, Biozentrum LMU München, Groβhaderner Str. 2–4, 82152 Planegg-Martinsried, Germany
b Biochemie und Physiologie der Pflanzen, Biozentrum LMU München, Groβhaderner Str. 2–4, 82152 Planegg-Martinsried, Germany
c LS Biochemie der Pflanzen, Ruhr-Universität Bochum, Universitätsstr. 150, 44780 Bochum, Germany

1 To whom correspondence should be addressed. E-mail joerg.nickelsen{at}lrz.uni-muenchen.de, fax 0049 89 21809974773, tel. 0049 89 2180 74773.

Biogenesis of photosynthetic pigment/protein complexes is a highly regulated process that requires various assisting factors. Here, we report on the molecular analysis of the Pitt gene (slr1644) from the cyanobacterium Synechocystis sp. PCC 6803 (Synechocystis 6803) that encodes a membrane-bound tetratricopeptide repeat (TPR) protein of formerly unknown function. Targeted inactivation of Pitt affected photosynthetic performance and light-dependent chlorophyll synthesis. Yeast two-hybrid analyses and native PAGE strongly suggest a complex formation between Pitt and the light-dependent protochlorophyllide oxidoreductase (POR). Consistently, POR levels are approximately threefold reduced in the pitt insertion mutant. The membrane sublocalization of Pitt was found to be dependent on the presence of the periplasmic photosystem II (PSII) biogenesis factor PratA, supporting the idea that Pitt is involved in the early steps of photosynthetic pigment/protein complex formation.

Key Words: Synechocystis • TPR protein • POR • thylakoid membrane • chlorophyll


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