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Molecular Plant 2009 2(6):1410-1424; doi:10.1093/mp/ssp095
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© The Author 2009. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS.

Alb4 of Arabidopsis Promotes Assembly and Stabilization of a Non Chlorophyll-Binding Photosynthetic Complex, the CF1CF0–ATP Synthase

Monique Benza,b, Thomas Balsc, Irene Luise Gügela,b, Markus Piotrowskic, Andreas Kuhnd, Danja Schünemannc, Jürgen Solla,b and Elisabeth Ankelea,b,1

a Department of Biologie I-Botanik, Biozentrum Ludwig-Maximillians-University Munich, Grosshadernerstrasse 2–4, D-82152 Planegg-Martinsried, Germany
b Munich Centre for Integrated Protein Science CIPSM, Ludwig-Maximillians-University Munich, Feodor-Lynen-Strasse 25, D-81377 Munich, Germany
c Department of Plant Physiology, Ruhr-University Bochum, D-44780 Bochum, Germany
d Department of Microbiology, University of Hohenheim, Garbenstrasse 30, D-70599 Hohenheim, Germany

1 To whom correspondence should be addressed at a. E-mail elis.ankele{at}lrz.uni-muenchen.de, fax +49-(0)89-74752, tel. +49-(0)89-2180-74760.

All members of the YidC/Oxa1/Alb3 protein family are evolutionarily conserved and appear to function in membrane protein integration and protein complex stabilization. Here, we report on a second thylakoidal isoform of Alb3, named Alb4. Analysis of Arabidopsis knockout mutant lines shows that Alb4 is required in assembly and/or stability of the CF1CF0–ATP synthase (ATPase). alb4 mutant lines not only have reduced steady-state levels of ATPase subunits, but also their assembly into high-molecular-mass complexes is altered, leading to a reduction of ATP synthesis in the mutants. Moreover, we show that Alb4 but not Alb3 physically interacts with the subunits CF1β and CF0II. Summarizing, the data indicate that Alb4 functions to stabilize or promote assembly of CF1 during its attachment to the membrane-embedded CF0 part.

Key Words: Alb4 • Alb3 • Arabidopsis • thylakoid membrane protein insertion • protein complex assembly • CF1CF0–ATP synthase


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