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Molecular Plant Advance Access published online on June 25, 2009
Molecular Plant, doi:10.1093/mp/ssp042
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© The Author 2009. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS.

Global Analysis of Gene Expression Profiles in Brassica napus Developing Seeds Reveals a Conserved Lipid Metabolism Regulation with Arabidopsis thaliana

Ya Niua,2, Guo-Zhang Wua,2, Rui Yea, Wen-Hui Lina, Qiu-Ming Shia, Liang-Jiao Xuea, Xiao-Dong Xub, Yao Lib, Yu-Guang Duc and Hong-Wei Xuea,1

a National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science (SIBS), Chinese Academy of Sciences, 300 Fenglin Road, 200032 Shanghai, China
b United Gene Holdings, Ltd. 3–5, 1111 ZhongshanBeier Road, 200092 Shanghai, China
c The Nature Products and Glycoconjugate Research Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 47 Zhongshan Road, 16023 Dalian, Liaoning, China

1 To whom correspondence should be addressed. E-mail hwxue{at}sibs.ac.cn, fax 00 86 21 54924060, tel. 0086 21 54924059.

In order to study Brassica napus fatty acid (FA) metabolism and relevant regulatory networks, a systematic identification of fatty acid (FA) biosynthesis-related genes was conducted. Following gene identification, gene expression profiles during B. napus seed development and FA metabolism were performed by cDNA chip hybridization (>8000 EST clones from seed). The results showed that FA biosynthesis and regulation, and carbon flux, were conserved between B. napus and Arabidopsis. However, a more critical role of starch metabolism was detected for B. napus seed FA metabolism and storage-component accumulation when compared with Arabidopsis. In addition, a crucial stage for the transition of seed-to-sink tissue was 17–21 d after flowering (DAF), whereas FA biosynthesis-related genes were highly expressed primarily at 21 DAF. Hormone (auxin and jasmonate) signaling is found to be important for FA metabolism. This study helps to reveal the global regulatory network of FA metabolism in developing B. napus seeds.

Key Words: Fatty acid • Brassica napus • seed development • starch • EST

2 These authors contributed equally to the studies.


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