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Molecular Plant Advance Access published online on August 26, 2009
Molecular Plant, doi:10.1093/mp/ssp069
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© The Author 2009. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS.

Metabolomic Screening Applied to Rice FOX Arabidopsis Lines Leads to the Identification of a Gene-Changing Nitrogen Metabolism

Doris Albinskya,2, Miyako Kusanoa,2, Mieko Higuchia, Naomi Hayashia, Makoto Kobayashia, Atsushi Fukushimaa, Masaki Morib, Takanari Ichikawaa, Keiko Matsuia, Hirofumi Kurodaa, Yoko Horiia, Yuko Tsumotoa, Hitoshi Sakakibaraa, Hirohiko Hirochikab, Minami Matsuia and Kazuki Saitoa,c,1

a RIKEN Plant Science Center, Tsurumi-ku, Yokohama 230-0045, Japan
b National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan
c Graduate School of Pharmaceutical Sciences, Chiba University, Inage-ku, Chiba 263-8522, Japan

1 To whom correspondence should be addressed. E-mail ksaito{at}psc.riken.jp, fax +81 45 503 9488, tel. +81 45 503 9489.

Plant metabolomics developed as a powerful tool to examine gene functions and to gain deeper insight into the physiology of the plant cell. In this study, we screened Arabidopsis lines overexpressing rice full-length (FL) cDNAs (rice FOX Arabidopsis lines) using a gas chromatography–time-of-flight mass spectrometry (GC–TOF/MS)-based technique to identify rice genes that caused metabolic changes. This screening system allows fast and reliable identification of candidate lines showing altered metabolite profiles. We performed metabolomic and transcriptomic analysis of a rice FOX Arabidopsis line that harbored the FL cDNA of the rice ortholog of the Lateral Organ Boundaries (LOB) Domain (LBD)/Asymmetric Leaves2-like (ASL) gene of Arabidopsis, At-LBD37/ASL39. The investigated rice FOX Arabidopsis line showed prominent changes in the levels of metabolites related to nitrogen metabolism. The transcriptomic data as well as the results from the metabolite analysis of the Arabidopsis At-LBD37/ASL39-overexpressor plants were consistent with these findings. Furthermore, the metabolomic and transcriptomic analysis of the Os-LBD37/ASL39-overexpressing rice plants indicated that Os-LBD37/ASL39 is associated with processes related to nitrogen metabolism in rice. Thus, the combination of a metabolomics-based screening method and a gain-of-function approach is useful for rapid characterization of novel genes in both Arabidopsis and rice.

Key Words: Metabolite profiling • GC–TOF/MS • rice FOX Arabidopsis lines • transcript profiling • LBD37/ASL39 • nitrogen metabolism

2 These authors contributed equally to this work.


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