Skip Navigation


Molecular Plant Advance Access originally published online on May 22, 2008
Molecular Plant 2008 1(3):423-445; doi:10.1093/mp/ssn019
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Data
Right arrow All Versions of this Article:
1/3/423    most recent
ssn019v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Denoux, C.
Right arrow Articles by Dewdney, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS.

Activation of Defense Response Pathways by OGs and Flg22 Elicitors in Arabidopsis Seedlings

Carine Denouxa,b, Roberta Gallettic, Nicole Mammarellaa, Suresh Gopalana, Danièle Werckb, Giulia De Lorenzoc, Simone Ferraric, Frederick M. Ausubela and Julia Dewdneya,1

a Department of Genetics, Harvard Medical School, and Department of Molecular Biology, Massachusetts General Hospital, 185 Cambridge St, Simches 7700, Boston, MA 02114, USA
b Institut de Biologie Moléculaire des Plantes, CNRS, Université Louis Pasteur, Strasbourg, France
c Dipartimento di Biologia Vegetale, Università di Roma ‘La Sapienza’, Piazzale Aldo Moro 5, 00185 Rome, Italy

1 To whom correspondence should be addressed. E-mail dewdney{at}molbio.mgh.harvard.edu, fax 617-643-3050, tel. 617-643-3325.

We carried out transcriptional profiling analysis in 10-d-old Arabidopsis thaliana seedlings treated with oligogalacturonides (OGs), oligosaccharides derived from the plant cell wall, or the bacterial flagellin peptide Flg22, general elicitors of the basal defense response in plants. Although detected by different receptors, both OGs and Flg22 trigger a fast and transient response that is both similar and comprehensive, and characterized by activation of early stages of multiple defense signaling pathways, particularly JA-associated processes. However, the response to Flg22 is stronger in both the number of genes differentially expressed and the amplitude of change. The magnitude of induction of individual genes is in both cases dose-dependent, but, even at very high concentrations, OGs do not induce a response that is as comprehensive as that seen with Flg22. While high doses of either microbe-associated molecular pattern (MAMP) elicit a late response that includes activation of senescence processes, SA-dependent secretory pathway genes and PR1 expression are substantially induced only by Flg22. These results suggest a lower threshold for activation of early responses than for sustained or SA-mediated late defenses. Expression patterns of amino–cyclopropane–carboxylate synthase genes also implicate ethylene biosynthesis in regulation of the late innate immune response.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.