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Molecular Plant Advance Access originally published online on December 9, 2008
Molecular Plant 2009 2(2):357-367; doi:10.1093/mp/ssn084
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© The Author 2008. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS.

Redox-Dependent Regulation of the Stress-Induced Zinc-Finger Protein SAP12 in Arabidopsis thaliana

Elke Ströher, Xin-Jia Wang, Nils Roloff, Peter Klein, Arne Husemann and Karl-Josef Dietz1

Biochemistry and Physiology of Plants, Bielefeld University, 33501 Bielefeld, Germany

1 To whom correspondence should be addressed. E-mail karl-josef.dietz{at}uni-bielefeld.de, fax +49 521 106 6039.

The stress-associated protein SAP12 belongs to the stress-associated protein (SAP) family with 14 members in Arabidopsis thaliana. SAP12 contains two AN1 zinc fingers and was identified in diagonal 2D redox SDS–PAGE as a protein undergoing major redox-dependent conformational changes. Its transcript was strongly induced under cold and salt stress in a time-dependent manner similar to SAP10, with high levels after 6 h and decreasing levels after 24 and 48 h. The transcript regulation resembled those of the stress marker peroxiredoxin PrxIID at 24 and 48 h. Recombinant SAP12 protein showed redox-dependent changes in quaternary structure as visualized by altered electrophoretic mobility in non-reducing SDS polyacrylamide gel electrophoresis. The oxidized oligomer was reduced by high dithiothreitol concentrations, and also by E. coli thioredoxin TrxA with low dithiothreitol (DTT) concentrations or NADPH plus NADPH-dependent thioredoxin reductase. From Western blots, the SAP12 protein amount was estimated to be in the range of 0.5 ng µg–1 leaf protein. SAP12 protein decreased under salt and cold stress. These data suggest a redox state-linked function of SAP12 in plant cells particularly under cold and salt stress.

Key Words: abiotic/environmental stress • cold acclimation • cell signaling • gene expression • Arabidopsis • A20 and AN1 Zinc finger domains • redox regulation • stress associated protein • thioredoxin


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